Coding

Part:BBa_K380010:Design

Designed by: Nina Schiller   Group: iGEM10_Stockholm   (2010-10-26)

Immunoglobulin G protease (IdeS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 268


Design Notes

The start and stop codon was removed and the gene was redesigned with BioBrick prefix and suffix according to assembly standard 25 using specifically designed primers and PCR.

Source

Organism: Streptococcus pyogenes serotype M1 http://www.uniprot.org/uniprot/Q7DAM2

References